Show simple item record

dc.contributor.authorHuang, Yihui
dc.contributor.authorZhou, Jun
dc.contributor.authorHakamivala, Amirhossein
dc.contributor.authorWu, Jinglei
dc.contributor.authorHong, Yi
dc.contributor.authorBorrelli Jr., Joseph
dc.contributor.authorTang, Liping
dc.date.accessioned2018-04-12T02:34:37Z
dc.date.available2018-04-12T02:34:37Z
dc.date.issued07 September 2017
dc.identifier.citationPublished in Scientific Reports 7, Article number 10906: 1-10, 2017en_US
dc.identifier.urihttp://hdl.handle.net/10106/27316
dc.description.abstractCartilage injury induced by acute excessive contact stress is common and mostly affects young adult. Although early detection of cartilage injury may prevent serious and lifelong arthritic complications, early detection and treatment is not possible due to the lack of a reliable detection method. Since chondrocyte injury and subsequent cell death are the early signs of cartilage injury, it is likely that cartilage cell apoptosis can be used to predict the extent of injury. To test this hypothesis, a near infrared probe was fabricated to have high affinity to apoptotic cells. In vitro tests show that this apoptosis probe has low toxicity, high specificity, and high affinity to apoptotic cells. In addition, there is a positive relationship between apoptotic cell numbers and fluorescence intensities. Using a mouse xiphoid injury model, we found significant accumulation of the apoptosis probes at the injured xiphoid cartilage site. There was also a positive correlation between probe accumulation and the number of apoptotic chondrocytes within the injured xiphoid cartilage, which was confirmed by TUNEL assay. The results support that the apoptosis probes may serve as a powerful tool to monitor the extent of mechanical force-induced cartilage injury in vivo.en_US
dc.description.sponsorshipWe thank Drs. Hong Weng and Chris Chen, and Ms. Qinglan Yang for technical assistance on cell culture and animal model, respectively. A portion of this work was supported by a grant from Texas Health Arlington Memorial Hospital. Part of this work was also supported by Translational Research Award No. W81XWH-14-1-0459 from the Defense Health Program through the Department of Defense Peer Review Orthopaedic Research Program.en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.rightsAttribution 4.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/us/*
dc.subjectCartilage injuryen_US
dc.subjectApoptosis -- Cartilage cellen_US
dc.subjectApoptotic cellsen_US
dc.titleAn optical probe for detecting chondrocyte apoptosis in response to mechanical injuryen_US
dc.typeArticleen_US
dc.publisher.departmentDepartment of Bioengineering, The University of Texas at Arlingtonen_US
dc.identifier.externalLinkDescriptionOriginal article available from article DOIen_US
dc.identifier.doiDOI:10.1038/s41598-017-10653-y


Files in this item

Thumbnail
Thumbnail


This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 United States
Except where otherwise noted, this item's license is described as Attribution 4.0 United States