Multifunctionality of indocyanine green-loaded biodegradable nanoparticles for enhanced optical imaging and hyperthermia intervention of cancer
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Date
2012Author
Nguyen, Kytai Truong
Patel, Ronak H.
Patel, Nimet L.
Kavuri, Venkaiah C.
Liu, Hanli
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The aim of this study was to develop and characterize multifunctional biodegradable and biocompatible
poly lactic-co-glycolic acid (PLGA) nanoparticles loaded with indocyanine green (ICG) as an optical-imaging contrast
agent for cancer imaging and as a photothermal therapy agent for cancer treatment. PLGA-ICG nanoparticles
(PIN) were synthesized with a particle diameter of 246 11 nm, a polydispersity index of 0.10 0.03, and ICG
loading efficiency of 48.75 5.48%. PIN were optically characterized with peak excitation and emission at 765
and 810 5 nm, a fluorescence lifetime of 0.30 0.01 ns, and peak absorbance at 780 nm. The cytocompatibility
study of PIN showed 85% cell viability till 1-mg∕ml concentration of PIN. Successful cellular uptake of ligand
conjugated PIN by prostate cancer cells (PC3) was also obtained. Both phantom-based and in vitro cell culture
results demonstrated that PIN (1) have the great potential to induce local hyperthermia (i.e., temperature increase
of 8 to 10°C) in tissue within 5 mm both in radius and in depth; (2) result in improved optical stability, excellent
biocompatibility with healthy cells, and a great targeting capability; (3) have the ability to serve as an image contrast
agent for deep-tissue imaging in diffuse optical tomography.