Publications - DO NOT EDIT
http://hdl.handle.net/10106/24283
2024-03-29T11:27:37Z
2024-03-29T11:27:37Z
Dual-Drug Containing Core-Shell Nanoparticles for Lung Cancer Therapy
Menon, Jyothi Unnikrishna
Kuriakose, Aneetta
Iyer, Roshni
Hernandez, Elizabeth
Gandee, Leah
Zhang, Shanrong
Takahashi, Masaya
Zhang, Zhang
Saha, Debabrata
Nguyen, Kytai Truong
http://hdl.handle.net/10106/27338
2024-01-09T16:53:00Z
0016-01-01T00:00:00Z
Dual-Drug Containing Core-Shell Nanoparticles for Lung Cancer Therapy
Menon, Jyothi Unnikrishna; Kuriakose, Aneetta; Iyer, Roshni; Hernandez, Elizabeth; Gandee, Leah; Zhang, Shanrong; Takahashi, Masaya; Zhang, Zhang; Saha, Debabrata; Nguyen, Kytai Truong
Late-stage diagnosis of lung cancer occurs ~95% of the time due to late manifestation of its symptoms, necessitating rigorous treatment following diagnosis. Existing treatment methods are limited by lack of specificity, systemic toxicity, temporary remission, and radio-resistance in lung cancer cells. In this research, we have developed a folate receptor-targeting multifunctional dual drug-loaded nanoparticle (MDNP) containing a poly(N-isopropylacrylamide)-carboxymethyl chitosan shell and poly lactic-coglycolic acid (PLGA) core for enhancing localized chemo-radiotherapy to effectively treat lung cancers. The formulation provided controlled releases of the encapsulated therapeutic compounds, NU7441 - a potent radiosensitizer, and gemcitabine - an FDA approved chemotherapeutic drug for lung cancer chemo-radiotherapy. The MDNPs showed biphasic NU7441 release and pH-dependent release of gemcitabine. These nanoparticles also demonstrated good stability, excellent hemocompatibility, outstanding in vitro cytocompatibility with alveolar Type I cells, and dose-dependent caveolaemediated in vitro uptake by lung cancer cells. In addition, they could be encapsulated with superparamagnetic iron oxide (SPIO) nanoparticles and visualized by MRI in vivo. Preliminary in vivo results demonstrated the low toxicity of these particles and their use in chemo-radiotherapy to
effectively reduce lung tumors. These results indicate that MDNPs can potentially be used as nanovehicles
to provide simultaneous chemotherapy and radiation sensitization for lung cancer treatment.
The authors would also like to acknowledge Dr. Mingyuan Wei for his help with LCST measurements and the Molecular Pathology Core at the University of Texas Southwestern Medical Center at Dallas for the assistance
with histology studies.
0016-01-01T00:00:00Z
A Dual-Modality System for Both Multi-Color Ultrasound-Switchable Fluorescence and Ultrasound Imaging
Kandukuri, Jayanth
Yu, Shuai
Cheng, Bingbing
Bandi, Venugopal
D’Souza, Francis
Nguyen, Kytai Truong
Hong, Yi
Yuan, Baohong
http://hdl.handle.net/10106/27314
2024-01-10T16:05:50Z
0004-01-01T00:00:00Z
A Dual-Modality System for Both Multi-Color Ultrasound-Switchable Fluorescence and Ultrasound Imaging
Kandukuri, Jayanth; Yu, Shuai; Cheng, Bingbing; Bandi, Venugopal; D’Souza, Francis; Nguyen, Kytai Truong; Hong, Yi; Yuan, Baohong
Gobalakrishnan, Sundaresan; Zweit, Jamal
Simultaneous imaging of multiple targets (SIMT) in opaque biological tissues is an important goal for molecular imaging in the future. Multi-color fluorescence imaging in deep tissues is a promising technology to reach this goal. In this work, we developed a dual-modality imaging system by combining our recently developed ultrasound-switchable fluorescence (USF) imaging technology with the conventional ultrasound (US) B-mode imaging. This dual-modality system can simultaneously image tissue acoustic structure information and multi-color fluorophores in centimeter-deep tissue with comparable spatial resolutions. To conduct USF imaging on the same plane (i.e., x-z plane) as US imaging, we adopted two 90 <degree>-crossed ultrasound transducers with an overlapped focal region, while the US transducer (the third one) was positioned at the center of these two USF transducers. Thus, the axial resolution of USF is close to the lateral resolution, which allows a point-by-point USF scanning on the same plane as the US imaging. Both multi-color USF and
ultrasound imaging of a tissue phantom were demonstrated.
0004-01-01T00:00:00Z
The Mechanisms and Biomedical Applications of an NIR BODIPY-Based Switchable Fluorescent Probe
Cheng, Bingbing
Bandi, Venugopal
Yu, Shuai
D’Souza, Francis
Nguyen, Kytai T.
Hong, Yi
Tang, Liping
Yuan, Baohong
http://hdl.handle.net/10106/27306
2024-01-10T15:25:41Z
0011-01-01T00:00:00Z
The Mechanisms and Biomedical Applications of an NIR BODIPY-Based Switchable Fluorescent Probe
Cheng, Bingbing; Bandi, Venugopal; Yu, Shuai; D’Souza, Francis; Nguyen, Kytai T.; Hong, Yi; Tang, Liping; Yuan, Baohong
Zweit, Jamal; Gobalakrishnan, Sundaresan; Cho, William Chi-shing
Highly environment-sensitive fluorophores have been desired for many biomedical applications. Because of the noninvasive operation, high sensitivity, and high specificity to the microenvironment change, they can be used as excellent probes for fluorescence sensing/imaging, cell tracking/imaging, molecular imaging for cancer, and so on (i.e., polarity, viscosity, temperature, or pH measurement). In this work, investigations of the switching mechanism of a recently reported near-infrared environment-sensitive fluorophore, ADP(CA)2, were conducted. Besides, multiple
potential biomedical applications of this switchable fluorescent probe have been demonstrated, including wash-free live-cell fluorescence imaging, in vivo tissue fluorescence imaging, temperature sensing, and ultrasound-switchable fluorescence (USF) imaging. The fluorescence of the ADP(CA)2 is extremely sensitive to the microenvironment, especially polarity and viscosity. Our investigations showed that the fluorescence of ADP(CA)2 can be switched on by low polarity, high viscosity, or the presence of protein and surfactants. In wash-free live-cell imaging, the fluorescence of ADP(CA)2 inside cells was found much brighter than the dye-containing medium and was retained for at least
two days. In all of the fluorescence imaging applications conducted in this study, high target-to-noise (>5-fold) was achieved. In addition, a high temperature sensitivity (73-fold per Celsius degree) of ADP(CA)2-based temperature probes was found in temperature sensing.
0011-01-01T00:00:00Z
Islet encapsulation - Strategies to enhance islet cell functions
Nguyen, Kytai Truong
Beck, Jonathan
Angus, Ryan
Madsen, Ben
Britt, David
Vernon, Brent
http://hdl.handle.net/10106/24522
2024-01-10T16:16:16Z
2007-01-01T00:00:00Z
Islet encapsulation - Strategies to enhance islet cell functions
Nguyen, Kytai Truong; Beck, Jonathan; Angus, Ryan; Madsen, Ben; Britt, David; Vernon, Brent
**Please note that the full text is embargoed** ABSTRACT: Diabetes is one of the most prevalent, costly, and debilitating diseases in the world. Although traditional
insulin therapy has alleviated the short-term effects, long-term complications are ubiquitous and harmful.
For these reasons, alternative treatment options are being developed. This review investigates one appealing
area: cell replacement using encapsulated islets. Encapsulation materials, encapsulation methods,
and cell sources are presented and discussed. In addition, the major factors that currently limit cell
viability and functionality are reviewed, and strategies to overcome these limitations are examined. This
review is designed to introduce the reader to cell replacement therapy and cell and tissue encapsulation,
especially as it applies to diabetes.
This is a copy of an article published in the Tissue Engineering ©2007[copyright Mary Ann Liebert, Inc.]; Tissue Engineering is available online at: http://online.liebertpub.com.
2007-01-01T00:00:00Z